Journal: Journal of immunological methods

Article Title: Quantification of the Epitope Diversity of HIV-1-Specific Binding Antibodies by Peptide Microarrays for Global HIV-1 Vaccine Development

doi: 10.1016/j.jim.2014.11.006

Figure Lengend Snippet: Composition of global HIV-1 peptide microarray.

Article Snippet: Peptide microarrays were produced using a non-contact high performance microarray printer on epoxy-modified slides (PolyAn; Germany).

Techniques: Microarray

Journal: Journal of immunological methods

Article Title: Quantification of the Epitope Diversity of HIV-1-Specific Binding Antibodies by Peptide Microarrays for Global HIV-1 Vaccine Development

doi: 10.1016/j.jim.2014.11.006

Figure Lengend Snippet: The signal distribution of a representative microarray is displayed, as well as the correlation between slide sub-arrays. This data is following incubation with serum from an HIV-1-infected subject. SA, subarray; Rq, R squared; Sl, slope; In, intersection with the y axis; MC2, two closest values between the 3 sub-arrays; MC2 1 and MC2 2, each component of MC2.

Article Snippet: Peptide microarrays were produced using a non-contact high performance microarray printer on epoxy-modified slides (PolyAn; Germany).

Techniques: Microarray, Incubation, Infection

Journal: Journal of immunological methods

Article Title: Quantification of the Epitope Diversity of HIV-1-Specific Binding Antibodies by Peptide Microarrays for Global HIV-1 Vaccine Development

doi: 10.1016/j.jim.2014.11.006

Figure Lengend Snippet: Top row: A, C, G, CRF02_AG; bottom row: B, D, CRF01_AE, and all other clades. The X-direction of each plot represents the sequence of gp120. Sequences segments included on the microarray are depicted in red. In the Y-direction all sequences for the respective clade from the alignment HIV1_ALL_2009_ENV_PRO.fasta are shown (total 2248). The average coverage (horizontally) for each used HIV-1 sequence is 50%. The evaluation of coverage was performed presuming the same length of all sequences for one protein or fragment within a given clade.

Article Snippet: Peptide microarrays were produced using a non-contact high performance microarray printer on epoxy-modified slides (PolyAn; Germany).

Techniques: Sequencing, Microarray

Journal: Journal of immunological methods

Article Title: Quantification of the Epitope Diversity of HIV-1-Specific Binding Antibodies by Peptide Microarrays for Global HIV-1 Vaccine Development

doi: 10.1016/j.jim.2014.11.006

Figure Lengend Snippet: The signal and noise distribution of data from a sample microarray is displayed, as well as the correlation between slide sub-arrays. Microarray data is following incubation with plasma from an HIV-1-infected subject. FDR, false discovery rate; SD, standard deviation.

Article Snippet: Peptide microarrays were produced using a non-contact high performance microarray printer on epoxy-modified slides (PolyAn; Germany).

Techniques: Microarray, Incubation, Clinical Proteomics, Infection, Standard Deviation